Abstract
Dadras, H., Nazer, A.H.K. and Namavari, M.M. 1996. Distribution of R-factor and β-lactamase production in ampicillin resistant strains of Enterobacteriaceae isolated from equines. J. Appl. Anim. Res., 10: 81–89.
The antibiotic resistant patterns, transmission of R-factor and determination of β-lactamase production in ampicillin resistant strains belonging to family Enterobacteriaceae were studied. A total of 694 isolates from the faeces of 400 horses and donkeys comprised mainly of E. coli (56.05%), Klebsiella (17.58%), Proteus (10.80%) and Enterobacter (7.21%). 189 strains of these organisms were resistant against one or more antibiotics. The maximum (in terms of number of isolates) resistance was observed against ampicillin and chloramphenicol, whereas, against kanamycin and nalidixic acid it was the minimum. 147 strains (77.77 percent) of resistant isolates were capable of transferring either a part or the entire resistance pattern to sensitive recipient strains (E. coli K12-Nar and E. coli K12-Amr). Multiple drug resistant strains were more effecient in transferring their resistance pattern than single drug resistant ones. The capillary method was able to detect beta-lactamase in 86.46 percent of the isolates while the acidometric method detected 61.65 percent isolates only.