Effect of Hexachlorophene on In vitro Mortality and Some Enzyme Systems of Swine Kidney Worm Stephanurus dentatus

Abstract

Kaushal, P., Majumdar, S., Singh, K. and Agarwal, S.K. 1997. The effect of hexachlorophene on in vitro mortality and some enzyme systems of swine kidney worm Stephanurus dentatus. J. Appl. Anim. Res., 11: 49–56.

Adult Stephanurus dentatus obtained from the kidney and nodulated fat found around the kidney of common Indian pig (Sus scrofa) were exposed to 10⁻⁴ and 10⁻⁵M hexachlorophene at 37 C. The mortality of the parasites was observed after an exposure of 30 minutes in 10⁻⁴ M and an hour in 10⁻⁵M hexachlorophene. The effect of compound was also studied on various enzyme systems in the homogenates of the treated worms. Hexachlorophene in the concentration of 10⁻⁴M inhibited oxaloacetate reduction by 51% and 78%, and malate oxidation by 24% and 29% whereas, 10⁻⁵ M concentration caused an inhibition of 41% and 54% oxaloacetate reduction and 11% and 17% malate oxidation in male and female S. dentatus, respectively. Acid phosphomonoesterase activity was inhibited by 28% in male and 37% in female parasites with 10⁻⁴M hexachlorophene. The same concentration reduced the alkaline phosphomonoesterase activity by 75% and 80% in males and females, respectively. 10⁻⁵M hexachlorophene also caused a significant reduction of these enzymes. However, cholinesterase activity did not show any change upon hexachlorophene administration.

Key words:

• Hexachlorophene
• mortality
• enzyme system
• swine kidney worm