Ultrastructural Characteristics of Alginate-Induced Reddish Purple Stained Cells (ARPC) in Mouse Peritoneal Cavity

Abstract

He, W., Fujiki, K. and Fujihara, N. 2001. Ultrastructural characteristics of alginate-induced reddish purple stained cells (ARPC) in mouse peritoneal cavity. J. Appl. Anim. Res., 20: 15–26.

To characterize microscopic features of the alginate-induced reddish purple-stained cells (ARPC) using electron microscope, one ml of sodium alginate solution (10 mg/ml) was injected into mice intraperitoneally. Mice were killed after the injection at various time intervals to collect the peritoneal fluid. The cells contained in the fluid were collected by centrifugation and subjected to cytological examinations. As a result, electron microscopy revealed that the vacuole-like organelles (VLO) contained very small amount of electron-dense substance. The substance was stained reddish purple with Wright solution. ELISA using anti-alginate monoclonal antibody and uronic acid determination indicated that the substance is not phagocytosed sodium alginate but ARPC-derived cellular materials. It is concluded that the ARPC is shown to be obviously cellular organelles.

Keywords:

• Mice
• reddish purple-stained cells (ARPC)
• electron microscopy
• antibody
• phagocytose