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Abstract
Anbarasan, K., Sharma, A.K., Singh, R.K., Deb, S.M. and Sharma, D. 2001. Genetic differentiation among goats using randomly amplified polymorphic DNA (RAPD) markers. J. Appl. Anim. Res., 20: 83–88.
To develop population specific markers using randomly amplified polymorphic DNA—ploymerase chain reaction (RAPD—PCR) in meat (Black Bengal and Non-descript Rohilkhand Local), milk (Barbari) and pashmina (Chegu) producing goats, a total of 35 random sequence decamer primers were tested. Nine primers generated polymorphism in one or more goat populations. Out of 117 bands scored, 46 (39%) were observed to be polymorphic. One of the primers (OPM-18) was found effective in differentiating the Pashmina (Chegu) from meat or milk type of goats. While the primer OPI-05 generated one band which might be specific for meat type goats i.e. Black Bengal and Non-descript (Local). However, population specificity and sequence homology for these bands need further investigation by hybridization, cloning and sequencing experiments.
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