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Abstract
Ha, J.K., Lee, S.S., Goto, M., Moon, Y.H. and Cheng, K.-J. 2002. Influence of Tween 80 on the enzyme distribution in rumen liquor and on the growth of rumen bacteria and fungi. J. Appl. Anim. Res., 21: 129–143.
The effect of the surfactant Tween 80 on the enzyme distribution and growth of pure cultures of rumen anaerobic non-cellulolytic and cellulolytic bacteria and monoceiitric and polycentric fungi was investigated. When Tween 80 was added to rumen fluid at the level of 0.05 and 0.1 per cent (v/v), the total and specific activities of most of the cell-free enzymes were significantly (P<0.01) increased, but those of cell-bound enzymes did not follow a definite trend. The growth rates of rumen noncellulolytic bacteria (Ruminobacter amylophilus, Megasphaera elsdenii, Prevotella ruminicola and Selenomonas ruminantium) were significantly (P<0.01) increased by the addition of Tween 80 at both the concentrations tested. However, the growth rate of rumen cellulolytic bacteria (Fibrobacter succinogenes, Ruminococcus albus, Ruminococcus flavefaciens and Butyrivibrio fibrisolvens) were generally not affected by Tween 80. In general, Tween 80 appears to affect gram-negative bacteria more than gram-positive bacteria; and non-cellulolytic bacteria more than cellulolytic bacteria. The growth rates of ruminal monoceiitric fungi (Neocallimastixpatriciarum Piromyces communis) and polycentric fungi (Orpinomyces joyonii and Anaeromyces mucronatus) were also significantly (P<0.01) increased by the addition of 0.05 per cent Tween 80. These results indicate that the addition of 0.05 per cent Tween 80 may greatly stimulate the release of some enzymes from microbial cells and also stimulate the growth rates of a range of anaerobic ruminal microorganisms and thus can be used as a potential feed additive for ruminants.