Antigenic Characteristics of Fowl Adenovirus-4 (FAV-4) Isolates from Inclusion Body Hepatitis-hydropericardium Syndrome of Chickens

Abstract

Kaur, G., Maiti, N.K. and Oberoi, M.S. 2002. Antigenic characteristics of fowl adenovirus-4 (FAV-4) isolates from inclusion body hepatitis-hydropericardium syndrome of chickens. J. Appl. Anim. Res., 22: 231–236.

Two-dimensional (crossed) immunoelctrophoresis of 10 FAV-4 isolates revealed 6 immunoprecipitate peaks with FAV-4 antiserum. Purified isolates and standard FAV-4 strain revealed 8 structural polypeptides in SDS-PAGE ranging between 18 to 116 KD in molecular weight. Out of the eight polypeptides, only six polypeptides between 35–166 KD molecular weight were found to be immunogenic in immunoblotting study. This indicated no antigenic differences in the FAV-4 isolates from IBH-HPS cases.

Keywords:

• FAV-4
• IBH-HPS
• antigens
• electrophoresis
• immunoblotting