Detection of Antibodies Against Trypanosoma evansi in Dromedary Camels by Competitive Inhibition Enzyme-Linked Immunosorbent Assay

Abstract

Shahardar, R.A., Rao, J.R. and Mishra, A.K. 2003. Detection of antibodies against Trypanosoma evansi in dromedary camels by competitive inhibition enzyme-linked immunosorbent assay. J. Appl. Anim. Res., 24: 41–48.

A competitive inhibition enzyme-linked immunosorbent assay (CI-ELISA) using monospecific (polyclonal) antisera raised in BALB/c mice against affinity purified parasite glycoprotein was standardized for detection of antisera to Trypanosoma evansi in camels. Coating of purified trypanosome glycoprotein antigen at 2 μg ml⁻¹ concentration followed by incubation with camel (test) sera (1:200) and parasite glycoprotein specific sera designed for competitive inhibition (1:1000) and peroxidase labeled antimouse IgG and IgM conjugate (1:2000) gave satisfactory results. A total of 90 sera samples from camels in surra'endemic area of Bikaner, Rajasthan were examined by CI-ELISA and 63 (70%) were found positive for T. evansi antibodies.

Keywords:

• Camels
• CI-ELISA
• Trypanosoma evansi