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Abstract
Das, S. and Singh, V.P. 2003. Multiplex PCR for differentiation of Mycoplasma mycoides cluster from other mycoplasma. J. Appl. Anim. Res., 24: 95–100.
A multiplex PCR was standardized for detection of various members of Mycoplasma mycoides cluster and their differentiation from other mycoplasma as well as some other bacteria. Using mycoplasma group specific primers GPO-1/MGSO and Mycoplasma mycoides cluster specific primers MC 323/MC 358 simultaneously, two amplified products of 1.5 kbp and 715 bp were found only in members of M. mycoides cluster whereas, a single band of 715 bp was detected in all other non cluster mycoplasma and acholeplasma while no amplified product was detected in any other bacterial strain tested.