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Abstract
Biswas, T.K., Bhattacharya, T.K., Narayan, A.D., Badola, S., Kumar, P., Kumar, S. and Sharma, A. 2003. Effect of growth hormone gene polymorphism on milk quality traits in crossbred cattle. J. Appl. Anim. Res., 24: 145–151.
The study was conducted on 1/2 HarianaX1/2 Holstein Friesian (FH cross), 1/2 Holstein Friesian × 1/4 Brown Swiss × 1/4 Hariana (FBH cross) and 1/2 Holstein Friesian × 1/4 Jersey × 1/4 Hariana (FJH cross) cattle to find out the effect of polymorphism at growth hormone gene on milk quality traits. A fragment of 223 bp spanning over fourth quarter of 4th intron and almost whole 5th exon except last triplet codon was amplified and digested with Alu I restriction enzyme. Two groups of genotypes namely, LL and LV along with two types of alleles (L and V) were observed in three lines of crossbred cattle where the frequency of LL genotype and L allele was found to be comparatively higher. The growth hormone gene polymorphism had significant effect (P≤0.05) on total and daily milk fat and protein yield in first three lactations. LL genotype produced highest amount of daily fat, protein and total fat yield, whereas, LV genotype produced highest amount of total protein yield in crossbred cattle.