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Original Articles

PCR-RFLP in Complement Component C5 and Interleukin 12 Genes in Indian Pigs

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Pages 101-105 | Received 16 Apr 2004, Accepted 07 Aug 2004, Published online: 11 Nov 2011
 

Abstract

Kumar, KG. and Kumar, S. 2004. PCR-RFLP in complement component C5 and interleukin 12 genes in Indian pigs. J. Appl. Anim. Res., 26: 101–105.

This study was conducted to find the presence of polymorphic sites, which have been previously reported in commercial breeds of pigs, in Indian pigs since these are relatively more resistant to diseases. The site covering the polymorphic site was amplified with gene specific primers and the amplicons were digested with specific restriction enzymes. IL 12p40 gene polymorphism in intron 4 was found to be highly polymorphic in Indian pigs similar to commercial breeds of pig, whereas C5 gene had only 2 polymorphic sites out of 4 reported earlier. The study indicated that European breeds and Indian pigs were genetically distant.

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