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Abstract
Gutiérrez. C., Mendoza, G.D., Ricalde, R., Melgoza, L.M. and Plata, F. 2005. Effect of exogenous amylase or glucoamylase dose on in situ ruminal digestion of corn and sorghum. J. Appl. Anim. Res., 27: 7–10.
Several processes have been developed to increase ruminal digestion of grains. Treatment of grains with exogenous amylase from Bacillus licheniformis and with glucoamylase from Aspergillus niger has increased ruminal digestion. An in situ assay was conducted to determine the effects of the dose of exogenous amylolytic enzymes on in situruminal digestibility (12 h incubation) of corn and sorghum. A complete generalized randomized block design was used, with incubations as blocking criteria. In situ dry matter digestion was increased (P<0.05) with enzyme additions (57.5% control; glucoamylase 75.8%; amylase 82.9%), showing a linear and quadratic effect (P<0.05) of enzyme doses on grain DM digestion. It was concluded that both amylolytic thermostable enzymes have the potential as a feed additive to improve ruminal digestibility of corn and sorghum.