Polymorphism of Growth Hormone Gene and its Association with Expected Milk Production Traits in Dairy Bulls

Abstract

Pal, A., Chakravarty, A.K., Bhattacharya, T.K. and Sharma, A. 2005. Polymorphism of growth hormone gene and its association with expected milk production traits in dairy bulls. J. Appl. Anim. Res., 27: 29–33.

To explore polymorphism in 4th exon, 4th intron and 5th exon of growth hormone gene in Karan Fries (KF) cattle and Murrah buffalo bulls and its association with expected milk producing ability, a 428 bp fragment was amplified and digested with Alu I restriction enzyme. Two alleles L and V corresponding to leucine and valine variant of growth hormone polypeptide and thus two genotypes LL and LV were identified in Karan Fries bulls, whereas only LL genotype was observed in Murrah bulls. Karan Fries bulls with LL genotype were found to have higher (P≤0.01) expected predicted difference with respect to milk production traits as compared to the LV heterozygotes.

Key words:

• Buffalo
• cattle
• expected predicted difference
• growth hormone gene
• milk production
• PCR-RFLP