Partial Genomic Sequence of Buffalo (Bubalus bubalis) ITGB2 Gene

Abstract

Niranjan, S.K., Deb, S.M., Mitra, A., Sharma, D., Sharma, A and Sharma A. 2005. Partial genomic sequence of buffalo (Bubalus bubalis) ITGB2 gene. J. Appl. Anim. Res., 28: 41–43.

A fragment of 367 bp corresponding to parts of fifth and sixth exons flanking intron five of ITGB2 was amplified in river buffaloes (Bubalus bubalis). The Taq I-RFLP analysis showed the absence of BLAD (D128G) allele in the buffalo population studied. The sequence data of the amplicon, first report on buffalo ITGB2, was submitted to Genbank. Single nucleotide changes at seven places in fifth exon of buffalo ITGB2 led to substitution of Val¹⁴⁰ and Asp¹⁵⁹ and creation of one Msp I site.

Keywords:

• Buffalo
• Bubalus bubalis
• ITGB2
• ß₂ integrin
• partial sequence
• BLAD