Circannual Identification and Quantification of Constitutive Heat Shock Proteins (HSP 70) in Goats

Abstract

Meza-Herrera, C.A., Martínez, L., Aréchiga, C., Bañuelos, R., Rincón, R.M., Urrutia, J., Salinas, H. and Mellado, M. 2006. Circannual identification and quantification of constitutive heat shock proteins (Hsp 70) in goats. J. Appl. Anim. Res., 29: 9–12.

The constitutive expression of the heat shock protein-70 (Hsp 70) in goat lymphocytes was analyzed. Goats (n=30, five per breed, 25±3 mo., 33+3 kg) included the breeds Granadina (G), Nubian (N), Saanen (S), Toggenburg (T), Alpine (A) and Crosses (C; Saanen x Criollo). Data on body weight (BW) and condition (BCS), maximum (Tmax) and minimum (Tmin) temperatures and monthly thermal oscillations (Tmax- Tmin) were collected in March, June, September and December. Quantification of Hsp 70 considered total protein determination and characterization by SDS-PAGE, Hsp 70 immunodetection and quantification by optic densitometry. The highest(P<0.001) Hsp 70 expression occurred in June and December, concomitant to the highest environmental temperature and the greatest temperature oscillation, respectively. While the highest (P<0.001) Hsp 70 expression was depicted by the G, N, S and T breeds, A and C depicted the lowest Hsp 70 expression. The biological trend shown by the A and C breeds suggest important adaptation ability, since they depicted the highest BW, the best BCS, as well as the lowest constitutive Hsp 70 expression.

Key words:

• Goats
• breeds
• Hsp 70
• environment