A Dot-Enzyme Immunoassay for Large Scale Screening of Sera for Antibodies to Mycobacterium avium subsp. paratuberculosis in Goats

Abstract

Rajukumar, K., Tripathi, B.N., Kurade, N.P. and Parihar, N.S. 2006. A dot-enzyme immunoassay for large scale screening of sera for antibodies to Mycobacterium avium subsp. paratuberculosis in goats. J. Appl. Anim. Res., 29: 49–52.

A simple and effective dot-enzyme immunoassay procedure for large scale screening of antibodies to Mycobacterium avium subsp. paratuberculosis was evaluated using sequential sera from goats experimentally infected with M. a. paratuberculosis. Field evaluation of the test was carried, out on 168 serum samples from three goat flocks. The diagnostic value of the dot-EIA was comparable to that of plate ELISA. The sensitivities and specificities for the dot-EIA were found to be 65.6% and 92.9% and for plate ELISA 78% and 89%, respectively. Significant differences were not found in the positive and negative predictive values of both tests. The test design is suitable for screening of sera for antibodies to M. a. paratuberculosis at the field level.

Keywords:

• Dot-EIA
• ELISA
• paratuberculosis
• goats