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Abstract
Ravindran, R, Mishra, A.K. and Rao, J.R. 2006. Methodology for raising high parasitaemic Babesia bigemina infection in donor bovine calves. J. Appl. Anim. Res, 29: 59–60.
For obtaining large number of piroplasms required for various laboratory tests a new method has been developed. Healthy bovine calves were experimentally infected with Babesia bigemina. After confirming infection, spleen was removed. On day 6 post splenectomy, dexamethasone was administered for 5 days for immunosuppression. This resulted in a parasitaema as high as 35 per cent. This method has been found to be superior to OIE (2000) recommended protocol.
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