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Abstract
Furuta, H., Ichikawa, E., Sugimura, S., Kikuchi, S., Yoshida, T., Mukouyama, H. and Tomogane, H. 2006. Gene transfer to mouse embryos by sperm mediated gene transfer method. J. Appl. Anim. Res., 29: 113–116.
To obtain transgenic mouse embryos by in vitro fertilization transgenic mouse sperm were produced by electroporation using green fluorescent protein (GFP)gene as a marker. The sperm was cultured in medium GFP gene. Electoroporation was carried out under 200 V-1μF, 200V-25μF and 200V-50μF. The survival rate of sperm decreased by each treatment. Although the DNA transfer into sperm mediated by electroporation was very easy, efficiency of introduction into embryos was very low. Sperm mediated gene transfer may be inferior to microinjection.