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Original Articles

Molecular Characterization of ompA Gene of Pasteurella multocida Serotype B: 2

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Pages 97-100 | Received 24 Mar 2006, Accepted 10 Dec 2006, Published online: 11 Nov 2011
 

Abstract

Singh, S., Singh, V.P., Kumar, A.A., Joseph, S., Cheema, P.S., Srivastava, S.K. and Singh, N. 2007. Molecular characterization of ompA gene of Pasteurella multocida serotype B: 2. J. Appl. Anim. Res., 31: 97–100.

The ompA gene of Pasteurella multocida serotype B: 2 (strain P52) was cloned in InsT/A vector for sequencing and gene sequence was compared with that of other strains (232, 95010872, T94289 and 95120769) of serogroup A. It revealed a 1077 nucleotide and it had additional 30 bp than that of serotype A: 3. About 11.5 to 14% divergence in ompA gene sequence was observed between serotype B: 2 and various strains of serogroup A of P. multocida. A variation of about 20% in the amino acid sequence of outer membrane protein A (OmpA) was also recorded between serogroups A and B.

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