Identification and Molecular Characterization of Cathelin-like Prepro Region from Bubalus bubalis

Abstract

Das, H., Sharma, B. and Kumar, A. 2007. Identification and molecular characterization of cathelin-like prepro region from Bubalus bubalis. J. Appl. Anim. Res., 31: 213–216.

In the present study primers from the conserved region of reported Bos taurus cathelicidins were designed and a cathelin-like prepro region of 273 bp from myeloid cells of water buffalo was amplified which codes for 10.5 kDa peptide of 91 residues. This prepropeptide shares 86.4% and 80.2% sequence identity at nucleotides and amino acids level, respectively, with the prepro region of the earlier reported buffalo cathelicidin amplified using primer designed from cattle indolicidin. The results are suggestive of the presence of a different cathelicidin congener in Bubalus bubalis.

Keywords:

• Cathelin
• antimicrobial peptide
• cathelicidin
• prepro region
• buffalo
• cloning