Comparison of Method of Application on the Effect of Amylolytic Enzymes on In vitro Ruminal Starch Digestion

Abstract

Rojo, R., Mendoza, G.D., Plata, F.X., Lara, A. and Bárcena, R. 2007. Comparison of method of application on the effect of amylolytic enzymes on in vitro ruminal starch digestion. J. Appl. Anim. Res., 32: 81–84.

An in vitro, experiment was designed to study the efficacy of two amylolytic enzymes on starch digestion of corn applied to grain 24 h before incubation or at the beginning of the fermentation. Both enzymes, amylase from B. licheniformis, and glucoamylase from A. niger, increased starch digestion and there was no effect of the aspersion of the enzyme before the incubation, or the addition of the solution during the fermentation. It is concluded that even when enzymes are soluble proteins, resistance to ruminál degradation is not improved by the contact with the grain before incubation.

Keywords:

• Amylase
• glucoamylase
• corn
• exogenous enzymes
• in vitro digestibility