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Abstract
Ravindran, R., Saravanan, B. C, Rao, J. R., Mishra, A. K., Bansal, G.C. and Ray, D. 2007. A PCR-RFLP method for specific detection of Theileria annulata. J. Appl. Anim. Res., 32: 111–112.
A polymerase chain reaction amplifying the ribosomal RNA gene from the genomic DNA was standardized and it amplified monomorphic ∼564 bp product from B. bigemina, T. annulata, and host leucocyte genomic DNA templates. Use of the enzyme kpn I resulted in restriction digestion only with T. annulata, specific PCR product yielding 466 and 98 bp fragments.
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