PCR-RFLP analysis of IL-2Rγ and IL-15Rα genes in Specialized Layer Chicken Lines

Abstract

Singh, A.P., Kumar, S. and Gupta, T. 2008. PCR-RFLP analysis of IL-2Rγ and IL-15Rα genes in specialized layer chicken lines. J. Appl. Anim. Res., 33: 29–31.

DNA polymorphism of IL-2Rγ and IL-15Rα was studied in 70 birds belonging to third generation of specialized White Leghorn chicken lines, divergently selected for humoral immune response to sheep erythrocytes by PCR-RFLP technique using specific primers. Amplification revealed product of 600bp for IL-2Rγ and 246bp for IL-15Rα genes. These amplicons digested with Hph I and Alu I revealed similar and monomorphic patterns in specialized layer chicken lines. It may be inferred that although specific primers amplified the target gene sequence but the PCR-RFLP pattern varied under different genetic backgrounds.

Keywords:

• Nucleotide polymorphism
• immune response
• chicken
• PCR
• RELP