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Abstract
Pedini, V., Dall'Aglio, C., Mercati, F., Pascucci, L. and Scocco, P. 2008. Glycoconjugates in sheep buccal glands investigated by conventional and lectin histochemistry. J. Appl. Anim. Res., 34: 49–54.
Sheep buccal glands consist of mucous acini capped by demilunes. Information on the chemical structure of their secretory glycoconjugates were obtained by means of a battery of peroxidase conjugated lectins with affinity for specific terminal and/or internal sugars. Neuraminidase procedures followed by lectin staining were also used to visualize the carbohydrate sequence. Stored secretions in mucous acinar cells contained glycoconjugates with N-acetylglucosamine and sialic acid linked to αN-acetylgalactosamine and galactosyl (βl→3) N-acetylgalactosamine. Demilunar cells displayed fucose, mannose, N-acetylglucosamine and α,N-acetylgalactosamine residues. Cells lining duct system showed a very strong staining at the apical surface and in the cytoplasm with UEA I, LTA and ConA.