Polymorphism on mtDNA D-loop and ND5 Regions Among Turkish Native Cattle Breeds Assessed by PCR-RFLP

Abstract

Ozdemir, M., Dogru, U. and Ciftci, M. 2009. Polymorphism on mtDNA D-loop and ND5 regions among Turkish native cattle breeds assessed by PCR-RFLP. J. Appl Anim. Res., 36: 37–40.

To determine the specific polymorphic sites of Turkish native cattle breeds (Turkish Grey, East Anatolian Red, South Anatolian Red and Anatolian Black) and genetic variations in mtDNA entire D-loop region with high mutation rate and 1100 bp of constant ND5 region were analyzed. In 910 bp of D-loop region, AluI, HaeIII, HpaII, AvaII, DraI and ApaI enzymes from ten restriction endonucleases, which have 17 restriction sites, showed polymorphic sites and 19 haplotypes were found. AluI, HaeIII, BglIí and SspI enzymes from ten restriction endonucleases, which have 25 restriction sites representing polymorphic sites, and 6 haplotypes were found in the ND5 region. Results of the cluster analysis carried out on the D-loop and ND5 region were similar to each other. East Anatolian Red, South Anatolian Red and Anatolian Black formed the common group distant from Turkish Grey.

Keywords:

• Animal resources
• mitochondrial DNA
• PCR-RFLP
• genetic diversity