Lack of Polymorphism in Partial Insulin Like Growth Factor 1 (IGF1) and Insulin Like Growth Factor Binding Protein 3 (IGFBP3) Genes of Mithun

Abstract

Panigrahi, M., Kumar, S., Deb, S.M., Mitra, A., Sharma, A. and Bujarbaruah, K.M. 2009. Lack of polymorphism in partial insulin like growth factor 1 (IGF1) and insulin like growth factor binding protein 3 (IGFBP3) genes of mithun. J. Appl. Anim. Res., 36: 41–44.

A 396 bp fragment encompassing exon 5 of Insulin like growth factor 1 (IGF1) gene and 652 bp fragment encompassing exon 2–3 of Insulin like growth factor binding protein 3 (IGFBP3) gene were digested by HindIII and NlaIII restriction enzymes, respectively, in 90 mithun (Bos frontalis). The PCR-RFLP analysis showed the absence of polymorphism in these fragments in the animals screened. HindIII restriction enzyme produced two fragments of 269 and 127 bp in exon-5 of IGF 1. Similarly NlaIII restriction enzyme produced two fragments of 551 and 101 bp in exon 2–3 ofIGFBP3 gene. Two single nucleotide differences in IGF1 while six such differences in IGFBP3 gene existed in mithun sequences when compared to cattle. The sequence of the amplicons, which were the first reports on these genes in mithun, were submitted to GenBank (Accession numbers EF686016 and EF686017, respectively).

Keywords:

• Bos frontalis
• exon 2–3
• exon 5
• HindIII
• IGF-1
• IGFBP-3
• mithun
• NlaIII
• PCR-RFLP