Cloning and Expression of the Porcine MEF2a Gene

Abstract

Zhang, D., Gao, Z., Liu, D. and Yang, G. 2009. Cloning and expression of the porcine MEF2a gene. J. Appl. Anim. Res., 36: 55–59.

To clone the complete coding sequence of the porcine MEF2a gene and to analyze its expression characterization, two isoforms of MEF2a gene (MEF2aA, MEF2aB) were obtained. Amino acid homology of two isoforms was 86.79%. It was completely identical in 1st-77th and 132th -470th amino acid, and was hypervariable in 78th-131st amino acid. MEF2aB had 34 amino acids (102bp) deletion in 224th-258th amino acid compared with MEF2aA. MEF2a was a broad-spectrum expression gene. Porcine MEF2aA, horse and house mouse existed in the same group, but porcine MEF2aB, cattle and human existed in another group in the phylogenetic tree. Therefore, MEF2a gene was presumed to be an apparent variation during evolutionary process.

Keywords:

• Porcine
• MEF2a gene
• clone
• expression
• muscle