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Abstract
Wang, Y., Fang, H., Cao, Y., Liu, L. and Jiang, M. 2010. Jintang black goat (Capra hircas) hnRNP K: molecular cloning, sequence analysis and expression detection. J. Appl. Anim. Res., 38: 159–164.
The cDNA encoding hnRNP K gene in Jintang Black goat was cloned by RT-PCR method. The entire cDNA sequence (1641 bp) contains a 1326 bp open reading frame and encodes 441 amino acids (48.709 kDa). Subsequent amino acid sequence analysis indicated that it showed a high level of sequence identity to Oryctolagus cuniculus (96.6%), Rattus norvegicus (94.5%), Bos taurus (99.7%), Homo sapiens (96.5%), Mus musculul (95.1%). Conserved domain and functional site analysis results showed that it has 3 KH domains, 3 RGG boxes, 1KNS signal, 1 acetylation site, was almost the same structure as hnRNP K proteins from other species. On the contrary, there is deletion mutation between 293 and 294, which amino acids sequence was “PGRGGRGGSRARNLPLPPPPP” in others species. Only 2 RGG-boxes (arginine-glycine- glycine boxes) were found, which always contains 5 RGG-boxes in hnRNP K protein from other species. Predicted tertiary structure results showed hnRNP K from Jintang Black goat belongs to A type, which has more turns in its third α-helix and this structure changes were caused by different C-terminus between A and B type of hnRNP K protein sequence. hnRNP K gene expression were detected in uterus, ovary, mammary gland, kidney, muscle but not in heart, pituitary and thalamencephalon. It means hnRNP K gene was mainly expressed in urogenital systems and may influence reproductive ability of Jintang black goat. The results of this study laid a solid foundation for subsequent function and structure research of hnRNP K in Jintang goat in the future.