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Abstract
Xian, G., Yaojing, Y., Shuo, J., Xueliang, C., Ruilin, F. and Xingxu, Z. 2010. Tandem inhibin gene immunization to induce sheep twinning. J. Appl. Anim. Res., 37: 93–96.
To study the effect of sheep twinning after tandem inhibin gene immunization, the recombinant plasmid of tandem inhibin were constructed with inhibin a-subunit (1–32) of pig and complement 3d of sheep by RT-PCR, which were used to immunize the sheep. The recombinant plasmid, pcDNA-DPPISS-DINH and pcDNA-DPPISS-DINH-sC3d3 were constructed successfully. After BHK-21 cells were transfected with the recombinant plasmid pcDNA-DPPISS-DINH and pcDNA-DPPISS-DINH-sC3d3, secreted expression was obtained. After immunization, the twinning rate of sheep was 12.5% and 25.0%, respectively, which was significantly higher (P<0.05) than the control group. The construction of recombinant plasmid of tandem inhibin gene made the theoretical and technical basis for developing the inhibin gene as vaccine for sheep.
