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Abstract
The objective of this study was to develop an accurate and reliable method of sex identification in the yak. To achieve this goal, a duplex-nested polymerase chain reaction (PCR) was optimised using DNA from male and female yaks and primers specific for SRY and bHBB gene sequences. Males showed both 1 SRY band and 1 bHBB band, but only 1 bHBB band was present in the agarose gel electrophoresis of females. The result of blind test for confirming the accuracy of the nested PCR showed all sex identifications were in agreement with the actual sexes of the yaks (26/26) from which blood samples were obtained. Thus, this study showed that this nested PCR method can be applied to sex the yak.
Acknowledgements
This research was funded by Science and Technology Bureau of Sichuan Province (No. 07JY029-128), PR China; and Southwest University for Nationalities (2011XWD-S071007).