ABSTRACT
Three serological methods used in the diagnosis of bovine brucellosis were conducted to assess the diagnostic sensitivity of the tests in brucellosis-infected herds. A total of 487 unvaccinated bovine serum samples collected from infected herds in the southeast region of Turkey having history of abortion with a known bacteriological status for the last three years were tested for anti-Brucella antibodies detection using the Rose Bengal plate test (RBPT), the complement fixation test (CFT) and indirect enzyme-linked immunosorbent assay (i-ELISA). A protein A/G conjugate was used in i-ELISA in order to reduce background reactivity and to use the test in multispecies if required. The serum samples were found as positive in 74.3%, 83.7% and 81.3% when we employed the CFT, i-ELISA and RBPT, respectively. i-ELISA could be used as a single test or combined with the RBPT for serologic diagnosis for brucellosis in infected herds.