In vitro antimicrobial activity of ozonated sunflower oil in milk against Escherichia coli: comparative study in cow, goat and sheep

ABSTRACT

Mastitis is one of the most impacting diseases in dairy farming. Conventional treatment of mastitis using antibiotics is costly and has led to the emergence of antimicrobial resistance against most of the commonly used antibacterial agents. Research has begun to focus on molecules with antimicrobial potentials structurally different from conventional antibiotics We compared the antibacterial activity in vitro of ozonized sunflower oil (OSO) with different peroxide concentrations (150, 300, and 600 PI) against E. coli in goat, cow and sheep milk. It was found that the antibacterial effect, after 72 h, was more important for the goat's milk with OSO 150 (p < 0.001) and OSO 300 (p < 0.001). However, the effect was greater for cow's milk, when OSO 600 was used (p < 0.001). In the case of sheep's milk, it was observed that the antimicrobial effect was only significant with the use of OSO 600, however, this decrease in the concentration of E. coli (p < 0.001) remained practically unchanged from 24 h to 72 h of incubation. In conclusion, ozonated sunflower oil offers many therapeutic possibilities that would reduce the use of antibiotics for the prevention or treatments of mastitis, and its antimicrobial effect is greater with cow's milk and less with sheep's milk.

KEYWORDS:

• cow
• goat
• sheep
• ozone
• milk

Acknowledgements

The authors would like to thank Mr. Juan Ramirez Verona from the Illustration and Iconography Service at Hospital Universitario de Gran Canaria Dr. Negrín, and Mr. Miguel Martín Betancor for their technical assistance during the preparation and design of figures and tables. The authors also thank Laboratorio Pérez del Toro (Gran Canaria, Spain) that provided the ozonated oil at different concentrations.

Disclosure statement

No potential conflict of interest was reported by the author(s).

Additional information

Funding

This work was funded in part by Fundación Canaria de Investigación y Salud, under grant PIFUN30/18, by Cabildo Insular de Gran Canaria, Spain under a grant CIGC2021 and by Spanish Ministry of Economy and Competitiveness under grant AGL2014-54683-R. The funding agencies had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.