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Abstract
The volatile constituents of Thymus daenensis growing wild in surrounding Shiraz- Iran were extracted by hydrodistillation (HD) and Head-Space Solid-Phase Micro Extraction (HS-SPME) methods and further analyzed by gas chromatography mass spectroscopy (GC-MS). In the HS-SPME and essential oil (EO) of T. daenensis, 40 and 28 components were identified respectively, which represented by 98.0 % and 99.7 % of the total detected components. The major volatile components in the HS and EO were thymol (9.1±0.83, 52.2 %), carvacrol (20.25±2.38, 37.4 %) and β-caryophyllene (17.98±2.19, 5.6 %) respectively. The concentration of thymol in the HS of the plant was determined to be 224±46 ng/L. The present paper shows similar major constituents detected by HS-SPME and HD-GC-MS, but the levels of the compounds are different in the above-mentioned extraction methods.