Abstract:
The volatile components from the fresh and dry leaves of three Pinus species (Pinus densiflora, P. thunbergii and P. rigida) was analyzed by headspace-gas chromatography-mass spectrometry. A total of eighty five compounds, representing 99.89 %, 99.61 %, 99.97 %, 99.86 %, 100 % and 99.69 % of the fresh and dry leaves of three Pinus species were identified. The major constituents from common components of the fresh leaves and dry leaves of the three Pinus plants were α-terpinolene (37.15 %, 36.59 %, 26.15 %, 28.6 %, 29.41 %, 34.76 %), 2-β-pinene (10.36 %, 9.52 %, 42.15 %, 41.89 %, 25.53 %, 32.75 %), α-pinene (3.19 %, 4.94 %, 0.44 %, 0.83 %, 0.51 %, 0.63 %), δ-4-carene (2.10 %, 1.40 %, 0.97 %, 0.78 %, 0.07 %, 0.05 %), trans-caryophyllene (0.87 %, 0.70 %, 1.62 %, 1.47 %, 0.48 %, 0.63 %). The results demonstrated that headspace-gas chromatography-mass spectrometry can be applied for extraction and separation of volatiles in Pinus plants, and the fresh leaves of P. thunbergii and P. densiflora can be used for medicinal resources.