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Abstract
The purpose of this study was to determine the chemical composition and antibacterial activity of the Moroccan Cedarwood essential oil. The sawdust samples of Cedrus atlantica were collected from Azrou, located in the heart of The Middle Atlas region of Morocco. The plant samples were subjected to hydrodistillation for eight hours. The oils were then analysed by Gas Chromatography equipped with flame ionization detector (GC-FID) and Gas chromatography coupled with Mass spectrometry (GC-MS). The antimicrobial activities were tested in vitro on gram-negative bacteria Escherichia coli and Salmonella, as well as gram-positive Staphylococcus aureus, Bacillus subtilis and Bacillus cereus using the agar diffusion assay/aromatogramme and minimum inhibitory concentration (MIC). Three bacterial strains (Escherichia coli, Bacillus subtilis and Bacillus cereus) were found to be sensitive to cedarwood essential oil and showed effective bactericidal activity with a Minimum Inhibitory Concentration of 0,4 μl/ml for Escherichia coli and Bacillus cereus, and 0,2 μl/ml for Bacillus subtilis.