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Abstract
The essential oil from the aerial parts of Leucas mollissima Wall. ex Benth growing wild in the Himalayan region of Uttarakhand, India was isolated by steam distillation method and analyzed by capillary GC and GC-MS. The oil was found to be rich in sesquiterpene hydrocarbons (64.1 %) followed by monoterpene hydrocarbons (13.7 %). Total thirty-five compounds were identified accounting for 87.1 % of the total oil composition. Germacrene D (16.0 %), valencene (15.6 %), β-caryophyllene (14.9 %), bicyclogermacrene (8.5 %), β-phellandrene (6.0 %) and ρ-cymene (4.6 %) were identified as the major constituents. The in vitro antibacterial activity of the oil was assessed using disc-diffusion method against ten bacterial strains. The oil showed significant activity against Gram-negative bacterium Agrobacterium tumefaciens and Gram-positive bacterium Bacillus subtilis. Minimum inhibitory concentration value was observed at 31.25 μL/mL against A. tumefaciens and B. subtilis.