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Abstract
The headspace (HS) composition of volatile compounds of leaf essential oil of medicinally important Morina longifolia Wallich (Wall.) was investigated by solid-phase microextraction/gas chromatography/flame ionization detector (SPME/GC/FID), and GC/mass spectrometry (MS). Forty six compounds (> 0.1 %) representing 96.20 % of the relative peak area of total chromatogram were detected. The oil was rich in sesquiterpene hydrocarbons with nineteen constituents amounting to 57.40 % of the oil with germacrene D (31.10 %) and α-humulene (5.50 %), as major constituents while trans-crysanthenyl acetate (4.50 %) and β-Phellandrene (3.60 %) were the main compounds in the monoterpene fraction (17.30 %). The results of study indicate the possible use of the volatile fraction of leaves essential oil of M. longifolia as a source of germacrene-D, a sesquiterpene of high perfumery value. The proposed HS-SPME method can also be applied to routine control analysis of aromatic and medicinal plants.