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Abstract
From hydro-distillation and GC-FID/MS (gas chromatography-flame ionization detection/mass spectrometry) analysis, chemical compositions in essential oils of two Vietnamese Piper plants Piper pendulispicum C. DC. and P. hymenophyllum Miq. were identified. Essential oils of P. pendulispicum leaf and stem are highlighted with the predominant amount of sesquiterpene hydrocarbons (73.21-78.06%), in which bicyclogermacrene (12.17-12.54%) and β-caryophyllene (12.10-14.51%) were the principal compounds. Monoterpene hydrocarbons (87.44%) are the main chemical class of P. hymenophyllum leaf oil, in which α-pinene (52.92%) and β-pinene (26.65%) are the principal compounds. These obtained essential oils with the MIC (minimum inhibitory concentration) values of 16-128 μg/mL are better than the positive control streptomycin ((MIC 128-256 μg/mL) in antimicrobial assay against three Gram-positive bacteria Enterococcus faecalis ATCC 29212, Staphylococcus aureus ATCC 25923, and Bacillus cereus ATCC 14579. P. pendulispicum essential oils (MIC 16-32 μg/mL) are also comparable to the other standard cycloheximide (MIC 32 μg/mL) against the yeast Candida albicans ATCC 10231. In general, P. pendulispicum essential oil is better than P. hymenophyllum leaf oil for antimicrobial treatment.