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Abstract
Tanacetum dolichophyllum (Kitam.) Kitam, commonly known as Tansy, belongs to the Asteraceae family. The plant species is an aromatic herb and is used by local inhabitants as incense. T. dolichophyllum samples were collected from Munsyari in the Pithoragarh district of Uttarakhand state in north India in the month of August. The essential oil was extracted separately from its flowers, leaves and roots by steam distillation and analyzed by GC and GC/MS. A total of 42 constituents from flower oil, 42 constituents from leaf oil and 38 constituents from root oil were identified constituting 85.3%, 82.8% and 96.0%, respectively of total oil composition. Flower oil of T. dolichophyllum was found to contain γ-eudesmol (24.5%), (E)-β- farnesene (7.2%), linalool (5.6%) and cis-p-menth-2-en-1-ol (4.8%) as the major constituents, whereas leaf oil was found to possess γ-eudesmol (20.3%), (E)-β- farnesene (13.4%) and E-caryophyllene (10.9%) as major compounds. The root oil of the plant species was dominated by (E)-2-(Hepta-2,4-diyn-1-ylidene)-1,6-dioxaspiro[4.4]non-3-ene [(E)-Tibetin spiroether] (58.3%), its Z-isomer (13.5%) and monoterpenes constituting 15.7% of the oil. The antibacterial activity of oil samples against ten different bacterial pathogens was determined by disc diffusion method. The oil samples from different parts of the plant showed different and moderate to good antibacterial activity.