Construction of a genetic map with SRAP markers and localization of the gene responsible for the first-flower-node trait in cucumber (Cucumis sativus L.)∗

∗Supported by the Shanghai Municipal Scientific & Technological Commission, (Grant No. 033107019) and Shanghai Municipal Agricultural Commission (Grant No. 2003-2-1-2)

Abstract

With, an F₂ population from the cross of two cucumber inbred lines, S06 and S52, sequence‐related amplified polymorphism (SRAP) was used to construct a genetic linkage map in cucumber (Cucumis sativus L.). Sixty-four SRAP primer combinations generated 108 polymorphic bands in the F₂ population analysis, The average of polymorphic bands produced by one primer pair was 1.5 and the maximum was 5. Using Mapmaker3.0, a linkage map was constructed, whhich consisted of 77 SRAP markers distributed in nine linkage groups (LOD≥3.0) and spanned 1114.2 cM with an average interval of 14.5 cM between markers. The gene for the first-flower-node trait, termed ffn, was mapped to linkage group IX, flanked by DC1EM5 and ME7EM2A at 10.3 cM and 12.1 cM distance, respectively

∗Supported by the Shanghai Municipal Scientific & Technological Commission, (Grant No. 033107019) and Shanghai Municipal Agricultural Commission (Grant No. 2003-2-1-2)

Keywords:

• cucumber(Cucumis sativus L.)
• SRAP (sequence-related amplified polymorphism)
• Linkage map
• First flower node

Notes

∗Supported by the Shanghai Municipal Scientific & Technological Commission, (Grant No. 033107019) and Shanghai Municipal Agricultural Commission (Grant No. 2003-2-1-2)