Modification of collagen-chitosan membrane by oxidation sodium alginate and in vivo/ in vitro evaluation for wound dressing application

Abstract

Collagen-chitosan (COL-CS) membranes materials without a cross-linking agent have poor mechanical properties. In this paper, COL-CS membranes were modified by a novel naturally-derived crosslinker, alginate dialdehyde (ADA) with different oxidation degree, and the COL-CS-ADA films were obtained. COL-CS-ADA films were characterized by Fourier transform attenuation total reflection infrared spectroscopy (ATR-FTIR), differential calorimetric scanning (DSC), thermogravimetric analysis (TG), tensile testing, and cross-link density testing. The modification of ADA exhibited positive effects on mechanical properties, the thermal stability of COL-CS membranes. The cross-linking degree between ADA and COL-CS membranes increased significantly with an increase in the oxidation degree. COL-CS-ADA films showed no cytotoxicity toward L929 fibroblasts and had good biocompatibility. The animal experiments showed that COL-CS-ADA film could promote wound healing.

Keywords:

• Collagen
• chitosan
• oxidation sodium alginate
• cross-linking
• in vivo/in vitro
• wound dressing

Additional information

Funding

This work was supported by the Open Fund of Fujian Provincial Key Laboratory of Green Design and Manufacture of Leather [2018XYL02] and the National Natural Science Foundation of China [51473001].