Determination of the Regiochemistry of D-glucal Glucosylation by Endo-ß-1,3-glucanase G_ACellulomonas cellulans using CI MS

Abstract

Enzymatic glycosylation of D-glucal was conducted by homogeneous endo-β-1,3-glucanase G_A from Cellulomonas cellulans in an aqueous environment. Water-soluble β-1,3-glucan (laminarin) was used as a polymeric donor of glycosyl residues. The single reaction product appeared to be monoglucosylated D-glucal as confirmed by FAB MS and MALDI MS analysis. The structure of the reaction product was determined to be D-laminarabial by comparison with CI MS spectra of two other possible regioisomers of β-monoglucosylated D-glucal and their peracetylated derivatives.

• Enzymatic Synthesis
• Transglycosylation
• Oligosaccharides
• Glycals
• Endo--1,3-glucanase
• Ci Ms