Abstract
The biotransformation of mulin-11,13-dien-20-oic acid (1), a diterpene isolated from extracts of Azorella compacta was evaluated using Aspergillus alliaceus UI 315 and Mucor circinelloides ATCC 7941. Cultures of A. alliaceus containing 1 produced the known metabolite 16-hydroxy-mulin-11,13-dien-20-oic acid (2) in 19.5% yield; alternatively, biotransformation of 1 by cultures of M. circinelloides produced two new metabolites identified as mulin-11,13-dien-16,20-dioic acid (3) and 7α,13β-dihydroxy-mulin-11-en-20-oic acid (4) on the basis of their spectroscopic data.
Acknowledgements
The authors wish to thank Prof. Pascal Richomme, University of Angers, France, for carrying ESI-MS and 1H/13C NMR analyses and HMBC experiment, as well as Karla Kú Durán, Erick Ic Caamal and Fabiola Escalante Erosa for technical support.
Disclosure statement
No potential conflict of interest was reported by the authors.