Type J CBFβ/MYH11 Transcript in the M4Eo Subtype of Acute Myeloid Leukemia

Abstract

Acute myeloid leukemia (AML) carrying inversion or translocation of chromosome 16 is usually associated with the FAB M4Eo morphological subtype and belongs to AMLs with a relatively favorable prognosis. At the molecular level, it is associated with a disease-specific fusion gene, CBFβ/MYH11. Previously, 10 different types of CBFβ/MYH11 fusion transcripts have been described in the literature, 7 of them are still known as unique cases. In the current study, peripheral blood and/or bone marrow samples from 265 AML patients were tested for the presence of the CBFβ/MYH11 fusion using RT-PCR and 12 (4.5%) positive cases were identified. The most common type A CBFβ/MYH11 transcript was confirmed in 11 patients. The transcript in the remaining one (a 71-year-old female) was different and sequence analysis allowed us to classify it as CBFβ/MYH11 type J. In contrast to the first type J case previously reported from Australia, this patient exhibited a typical FAB M4Eo morphology. The evidence of the second case indicates that the type J breakage might be a non-random event within the MYH11 gene.

Keywords:

• Inversion/translocation of chromosome 16
• FAB AML M4Eo
• RT-PCR
• CBFβ/MYH11 fusion transcript