Hormones in Foods: Presence of Enterostatin-Like Immunoreactivities in Bovine Milk

Abstract

Enterostatins, pentapeptides (Val-Pro-Asp-Pro-Arg [VPDPR], Val-Pro-Gly-Pro-Arg, Ala-Pro-Gly-Pro- Arg [APGPR], and others) derived from the amino terminus of procolipase, are endogenous to a variety of tissues and body fluids including brain, gut, blood, cerebrospinal fluid, and urine. The administration of exogenous peptides has been shown to elicit a variety of biologic activities, including a decrease in dietary fat preference and pancreatic insulin secretion. Since milk is a rich source of a variety of bioactive substances, especially peptides, we investigated the presence of enterostatin-like immunoreactivity in bovine milk. We measured enterostatins—APGPR and VPDPR—in milk from a herd of 19 cows randomly selected from the Louisiana State University Department of Dairy Science Research Herd in Baton Rouge; the results of this study show a mean peptide concentration in raw milk of 33.7 ± 2.9 ng/ml for APGPR and of 104.5 ± 16.3 ng/ml for VPDPR. A further chromatographic characterization of the nature of APGPR- and VPDPR-like immunoreactivities suggested the endogenous peptides share a common epitope with APGPR or VPDPR but are not APGPR or VPDPR. Unlike APGPR or VPDPR, the endogenous peptides were heat-labile and therefore their values were much lower in pasteurized milk.

Keywords:

• Cow's milk
• Enterostatins
• Val-Pro-Asp-Pro-Arg
• Val-Pro-Gly-Pro-Arg
• Ala-Pro-Gly-Pro-Arg