Isoflavonoid glycosides from the flowers of Pueraria lobata

Abstract

Pueraria lobata flower is a medicinal herb for treating intoxication, hepatic, and gastrointestinal tract lesions induced by alcohol. This study aims to investigate the isoflavonoid glycosides in P. lobata flowers. Two new isoflavone compounds were isolated from the extract of P. lobata flowers. Their structures were determined to be 5,6,7,4′-tetrahydroxyisoflavone-6,7-di-O-β-d-glucopyranoside and 5,6,7-trihydroxy-4′-methoxyisoflavone-6,7-di-O-β-d-glucopyranoside on the basis of spectroscopic means including HR-ESI-MS, UV, IR, ¹H, and ¹³C NMR.

Keywords:

• Pueraria lobata
• Leguminosae
• isoflavonoid
• 5,6,7,4′-tetrahydroxy isoflavone-6,7-di-O-β-d-glucopyranoside
• 5,6,7-trihydroxy-4′-methoxyisoflavone-6,7-di-O-β-d-glucopyranoside

Acknowledgements

Financial support was received from the National Major Projects for Science and Technology Development from Science and Technology Ministry of China (Grant No. 2009ZX09304-003), the Ministry of Science and Technology (2004CB518902), and Science and Technology Commission of Shanghai Municipality (036505003). The authors thank the Department of Analytical Chemistry of SIMM for all spectral measurements. The authors are grateful to Prof. Jin-Gui Shen of the Shanghai Institute of Materia Medica, Chinese Academy of Sciences, for the identification of the plant material.