Microbial transformation of deoxyandrographolide by Fusarium graminearum AS 3.4598

Abstract

Biotransformation of deoxyandrographolide (1) by Fusarium graminearum AS 3.4598 was investigated in this paper. And five transformed products of 1 by F. graminearum AS 3.4598 were obtained. Their chemical structures were characterized as 3-oxo-8α,17β-epoxy-14-deoxyandrographolide (2), 3-oxo-14-deoxyandrographolide (3), 3-oxo-17,19-dihydroxyl-8,13-ent-labdadien-15,16-olide (4), 1β-hydroxyl-14-deoxyandrographolide (5), and 7β-hydroxyl-14-deoxyandrographolide (6) by spectral methods including 2D NMR. Among them, products 2, 4, and 5 are new.

Keywords:

• microbial transformation
• deoxyandrographolide
• hydroxylation
• ent-labdane

Acknowledgements

We thank the National Natural Science Foundation of China (No. 81073013), the Important New Drug Research Project (2009ZX09502-025), the Science and Technology Development Common Project (KM201110858001), and the Scientific Research Base Program by the Beijing Municipal Commission of Education and PHR (IHLB) for financial support.