Bufadienolides and polyhydroxycholestane derivatives from Bufo bufo gargarizans

Abstract

A new polyhydroxycholestane sulfate ester, 3α,12β,25,26-tetrahydroxy-7-oxo-5β-cholestane 26-O-sulfate (1), was isolated from dried skin of Bufo bufo gargarizans Cantor and its structure was elucidated on the basis of extensive 1D and 2D NMR as well as HR-ESI-MS analysis. A comparison of steroidal metabolite profiles, based on HPLC and LC–MS analyses, indicates that the chemical compositions of the various parts of toads, such as venom, skin and stratum corneum, are significantly different.

Keywords::

• bufadienolide
• polyhydroxycholestane sulfate
• toad venom
• toad skin
• Bufo bufo gargarizans

Additional information

Funding

This work was financially supported by NSFC [grant number 30801512] and the Fundamental Research Funds for the Central Public Welfare Research Institutes [grant number ZZ070820] and [grant number Z02073].