Establishment of indirect enzyme-linked immunosorbent assay for artificial musk

Fang ShenState Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing100050, China

Jin-Ye BaiState Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing100050, China

Yu Meng Beijing Lianxin Pharmaceutic Co., Ltd., Beijing102628, China

Xuan XiaoState Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing100050, China; Beijing Lianxin Pharmaceutic Co., Ltd., Beijing102628, China

Shu ZhangState Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing100050, China; Beijing Lianxin Pharmaceutic Co., Ltd., Beijing102628, China

Xiu-Yuan ZhuState Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing100050, China

Gui-Fang ChengState Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing100050, ChinaCorrespondence[email protected]

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Abstract

Anti-inflammatory component (AIC) known as an important constituent of artificial musk exhibits bioactive effects on many pharmacological models. This study describes an immunochemical assay for the quality control of artificial musk in traditional Chinese medicine using an enzyme-linked immunosorbent assay. A polyclonal antibody against AIC was produced by rabbit. The method, at an effective measuring range of 3.13–200 ng/ml of AIC, 3 ng/ml limit of detection, and no cross-reaction with natural musk, successfully detected artificial musk in many traditional Chinese prescriptions containing artificial musk. The results demonstrated that a novel and reliable assay system for detection of artificial musk was generated.

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Establishment of indirect enzyme-linked immunosorbent assay for artificial musk

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Establishment of indirect enzyme-linked immunosorbent assay for artificial musk

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