Actinotetraose L, a new tetrasaccharide derivative isolated from the mycelia of Amycolatopsis sp. HCa1

Abstract

A new tetrasaccharide derivative, actinotetraose L (1), was isolated from the methanol extract of the mycelia of the grasshopper-associated rare actinobacterium Amycolatopsis sp. HCa1. The structure of the new compound was elucidated by a combination of 1D, 2D NMR (HMQC, HMBC, COSY, and NOESY), and HR-ESI-MS analyses as O-{3,4-di-O-[(E)-2-ethyl-2-butenoyl]- β-D-glucopyranosyl-(1 → 2)}-O-{3,4-di-O-[(E)-2-methyl-2-butenoyl]-β-D-glucopyranosyl-(1 → 2)}-6,6-di-O-[(E)-2-methyl-2-butenoyl]-α-D-glucopyranosyl-(1 → 1)-α-D-glucopyranoside. The immunosuppressive activity and cytotoxicity of the compound were evaluated by T-cell viability and MTT assays. Nonetheless, no significant activity was observed.

Keywords::

• Amycolatopsis
• Pseudonocardiaceae
• grasshopper
• tetrasaccharide

Disclosure statement

No potential conflict of interest was reported by the author(s).

Additional information

Funding

This work was co-financed by the National Natural Science Foundation of China [grant number 81172948], [grant number 81121062], [grant number 21132004], and [grant number 41406083], the 863 Project (2013AA092903), the Project for New Century Excellent Talents in University [grant number NCET-10-0477], the Hainan Natural Science Foundation [grant number 313079], and the National Nonprofit Institute Research Grant of CATAS-ITBB [grant number ITBB140405].