Regio-selective deglycosylation of icariin by cell suspension cultures of Glycyrrhiza uralensis and Morus alba

Abstract

Biotransformations of icariin (1) by cell suspension cultures of Glycyrrhiza uralensis and Morus alba yielded two new metabolites, icaruralins A and B (2 and 3), and one known metabolite, baohuoside I (4). Their structures were determined on the basis of extensive spectroscopic analysis. This is the first report that the cell suspension cultures of G. uralensis and M. alba possess deglycosylation functionality.

Keywords::

• icariin
• cell suspension cultures
• regio-selective deglycosylation
• Glycyrrhiza uralensis
• Morus alba

Disclosure statement

No potential conflict of interest was reported by the author(s).

Supporting information available

The HPLC-UV analysis of biotranformation of 1, HR-ESI-MS, IR, CD, 1D, and 2D NMR spectra of 2–3, the ¹H NMR and ¹³C NMR spectra for 4. This material is available free of charge via the Internet at http://www.tandfonline.com/toc/ganp20/current

Notes

^1. These authors contributed equally to this work.

Additional information

Funding

This work was financially supported by the National Natural Science Foundation of China [grant number 81273405]; the National Science & Technology Major Project “Key New Drug Creation and Manufacturing”, China [grant number 2012X09301002-001-005].